The science team at Spyryx has recently published an open-access article in the Journal of Cystic Fibrosis, supporting the stability of SPX-101 in the protease-rich environment of cystic fibrosis airways. Here, we demonstrate SPLUNC1, the natural allosteric regulator of ENaC, is degraded by multiple proteases present in sputum of CF patients. SPX-101 is stable in CF sputum and after exposure, retains functionality to internalize ENaC, raise the apical hydration of CF cells, and promote the survival of a mouse model of CF. This is not the case for the natural sequence of the ENaC-regulatory fragment of SPLUNC1, which is rapidly degraded under the same conditions.
Read the full open-access article here: https://www.cysticfibrosisjournal.com/article/S1569-1993(18)30627-1/fulltext